Gene Expression Core

The ability to survey expression of large parts of the genome is increasingly appearing to be a useful technique for gaining insights into the basic biology of aging. The specific aims of the core are to facilitate access by our biology of aging faculty to gene expression and proteomics technologies.

Transcriptomics:

• Assistance in preparing, labeling and quality control of RNA for expression studies.
• Use of expression arrays. Most work is performed with oligonucleotide arrays, such as those from Affymetrix. The cost of using arrays for research in the Basic Biology of Aging is generally subsidized by approximately 50%.
• Analysis of array data, including state-of-the-art statistical and analytical software applications and consulting by Dr. Dick Beyer.
• Assistance with validation of expression data by real time PCR.

Proteomics:

• Dr. MacCoss provides expertise to Biology of Aging investigators in proteomics, with specialization in isotopic labeling for quantitative proteomics, 'shotgun' proteomics, and tandem mass spectrometry. He will help guide Nathan Shock investigators in methods and applications of these technologies to the biology of aging.

For additional information, please contact Dr. Mike MacCoss (proteomics) at maccoss@u.washington.edu or Dr. Katy Gollahon (transcriptomics) at gollahon@u.washington.edu.