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Dr. Hudson has been working to survey gene expression changes with age in human heart, skeletal
muscle, skin and stomach tissues in tissues collected by Lifespan Biosciences as part of an SBIR
grant collaboration. To date all sample RNAs have been labeled and hybridized to Affymetrix
GeneChips and the data collected. Currently she is in the process of assessing and analyzing the
data set. Additionally, in the past year Dr. Hudson has completed sample preparation and microarray
hybridization of RNA made available from mouse studies in other labs involving GHRKO and Ames dwarf
mice to evaluate gene expression patterns in various models of lifespan extension. Although data
analysis is ongoing, some real-time PCR validation of candidate genes has been accomplished.
Finally, work is continuing on the development of BAC transgenic mice overexpressing glutamate
cysteine ligase modifier subunit, superoxide dismutase, and mitochondrially-expressed catalase.
Publications:
1. Bea F, Hudson FN, Chait A, Kavanagh TJ, and Rosenfeld ME. Induction of glutathione synthesis in
macrophages by oxidized low-density lipoproteins is mediated by consensus antioxidant response
elements. Circ Res. 2003;92:386-393.
2. Bea F, Puolakkainen, McMillen T, Hudson FN, Mackman N, Kuo CC, Campbell LA, and Rosenfeld ME.
Chlamydia pneumoniae induces tissue factor expression in mouse macrophages via activation of Egr-1
and the MEK-ERK1/2 pathway. Circ Res. 2003;92:394-401.
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